ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the histopathological characteristics and clinical implication of sarcolemma tissue in prepubertal concealed penis.</p><p><b>METHODS</b>After measurement of the penile length, 10 prepubertal children with congenital concealed penis underwent modified Devine's operation (treatment group), and another 10 normal prepubertal children received circumcision (control group). The anatomic features of the penile sarcolemma tissue was observed intraoperatively, and its fibrosis was evaluated by Masson trichrome staining.</p><p><b>RESULTS</b>The penile length of the treatment group was significantly shorter than that of the control group preoperatively ([1.49 +/- 0.17 ] cm vs [4.26 +/- 0.23 ] cm, P < 0.01). The degree of penile concealment was correlated with the distal point of the attachment of its sarcolemma fibrous tissue: the closer the distal attachment point was to the coronary ditch, the more serious was penile concealment. The proportion of the area of collagen fibers in the penile sarcolemma tissue was significantly higher in the treatment group than in the control ([65.6 +/- 6.9]% vs [37.1 +/- 4.7]%, P < 0.01).</p><p><b>CONCLUSION</b>Sarcolemma fibrosis was obvious in congenital concealed penis, and the key to its management is drastic removal of all the fibrous sarcolemma tissue.</p>
Subject(s)
Child , Humans , Male , Circumcision, Male , Fibrosis , Penis , Congenital Abnormalities , Pathology , General Surgery , Phimosis , Pathology , General Surgery , Sarcolemma , PathologyABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of hypothermia on the antioxidant capacity of rat testes after testicular torsion.</p><p><b>METHODS</b>Twenty-four healthy pubertal male Sprague-Dawley rats were randomly divided into three groups of equal number: Group A (torsion) , Group B (torsion + hypothermia) and Group C (control). The animals were submitted to unilateral 720 degrees testicular torsion, and underwent detorsion two hours later. Fourteen days later, the total antioxidant capacity (T-AOC) and the level of malonic diethylaldehyde(MDA) were detected with spectrophotometer and histological changes were observed by light microscope.</p><p><b>RESULTS</b>The T-AOC of Group B was significantly greater than that of Group A (P < 0.01), but less than that of Group C (P < 0.01). The MDA level of Group B was lower than that of Group A (P < 0.01), but higher than that of Group C (P < 0.05).</p><p><b>CONCLUSION</b>Hypothermia can restrain the production of oxygen free radicals following testicular torsion/detorsion in rats, which in turn can inhibit lipid peroxidation and increase the survivability of the torsional testis.</p>